Determination of the molecular-genetic mechanisms of cellular plasticity and pancreatic tissue remodeling in SHR rats

Abstract

Essential arterial hypertension is accompanied by systemic neurohumoral and microcirculatory disturbances, oxidative stress, and metabolic alterations that may affect the endocrine compartment of the pancreas and promote its remodelling. A key component of these changes is endocrine cell plasticity, which is mediated by reprogramming of transcriptional networks and may contribute to the development of islet dysfunction. In spontaneously hypertensive rats, a model relevant to essential arterial hypertension, the molecular and genetic mechanisms underlying these processes remain insufficiently defined, which justifies analysing the expression profile of key genes associated with cellular plasticity and pancreatic tissue remodelling. The aim of the work: To determine the molecular mechanisms of cellular plasticity and pancreatic remodelling under conditions of essential arterial hypertension by analysing the expression profile of key genes. Materials and methods. For the analysis of gene expression, the real-time reverse transcription polymerase chain reaction method was used using the PARN-405Z RT² Profiler™ PCR Array Rat Stem Cell kit (QIAGEN, Germany), where the pancreas was the object of the study in experimental animals.