Changes in the expression of molecular genetic regulators of the functional state of endocrine cells in SHR rats

Abstract

Essential arterial hypertension is accompanied by chronic vascular and neurohumoral stress and microcirculatory disturbances that can alter the functional state of the endocrine compartment of the pancreas and promote its remodelling. The molecular mechanisms underlying these changes under conditions of hereditary hypertension remain insufficiently defined, particularly with respect to the involvement of vasoactive, inflammatory, and hypoxia-associated gene networks. In this context, SHR rats represent a relevant model of hereditary hypertension for a systematic assessment of expression changes in key hypertension-related genes in pancreatic tissue. The aim of the work: to determine changes in the expression of key molecular and genetic regulators that characterise the functional state of pancreatic endocrine cells in SHR rats under conditions of hereditary hypertension. Materials and methods. For the analysis of gene expression, the real-time reverse transcription polymerase chain reaction method was used using the PARN-037Z RT² Profiler™ PCR Array Rat Hypertension (QIAGEN, Germany), where the pancreas was the object of the study in experimental animals.