Some Aspects of Neuroprotective Action of a New Derivative of 3-Methylxanthine (Compound C-3) Under Conditions of Acute Disorder of Cerebral Circulation (ADCC) Modeling by Ischemic Stroke Type

Abstract

The purpose of the research was to study the antioxidant and neuroprotective properties of 8-benzilaminotheophyllinil-7-acetic acid (Compound C-3), as well as peculiarities of its action and advantages over the reference drugs. The work was performed on 80 white nonlinear rats of both sexes weighing 160-170 g. Bilateral ligation of the common carotid arteries was performed under thiopental (40 mg/kg) anesthesia. The investigated compound С-3 in a dose of 100 mg/kg and reference drugs in doses recommended by earlier studies (thiotriazolin 50 mg/kg, mexidol 200 mg/kg and 100 mg/kg citicoline) were administered endogastrically with a metal feeding tube for 4 days. Acute disorder of cerebral circulation (ADCC) modeling have led to disturbance in the system NO (increase in NOS activity and NO hyperproduction), deprivation in the activity of antioxidant enzymes (SOD, catalase and glutathione peroxidase) in the brain of experimental animals, and as a result, the activation of oxidative and nitrosative stress: increase in level of carbonylated proteins – aldehyde phenylhydrazone (APH) and ketone phenylhydrazone (KPH), markers of free radicals activation, and nitrotyrosine.